Part:BBa_K4728007:Design

PhaF

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 71
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 82
  Illegal NgoMIV site found at 630
  Illegal NgoMIV site found at 657
  Illegal NgoMIV site found at 672
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The sequence was codon optimized to Escherichia coli and made compatible with Type IIS RFC[1000] and BioBrick RFC[10].

Source

The sequence comes from the genome of Pseudomonas putida.

References

Maestro, Beatriz, et al. “A New Family of Intrinsically Disordered Proteins: Structural Characterization of the Major Phasin PhaF from Pseudomonas Putida KT2440.” PLoS ONE, vol. 8, no. 2, 15 Feb. 2013, p. e56904, https://doi.org/10.1371/journal.pone.0056904. Accessed 7 Feb. 2023.

Jendrossek, Dieter, and Daniel Pfeiffer. “New Insights in the Formation of Polyhydroxyalkanoate Granules (Carbonosomes) and Novel Functions of Poly(3-Hydroxybutyrate).” Environmental Microbiology, vol. 16, no. 8, 21 Jan. 2014, pp. 2357–2373, https://doi.org/10.1111/1462-2920.12356. Accessed 18 Nov. 2020.